An agar plate is a sterile Petri dish that contains agar plus nutrients, and is used to culture microorganisms. Generally, selecting substances are also added to the plate, such as antibiotics. Agar plate - Preparation of agar plates. Most types of agar are purchased pre-prepared in powder form, although it is possible to buy a base agar mix and add nutrients separately. They are dissolved in distilled water as per their instructions. It is usually necessary to gently boil the mixture to facilitate dissolvi ...

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Agar plate - Preparation of agar plates Agar plate - Inoculation techniques
Agar plate - Streaking Agar plate - Christmas tree Agar plate - Stab culture Agar plate - Preparing a lawn
Agar plate - Incubation of agar plates Agar plate - Types of agar plates Agar plate - Safe disposal of agar plates

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Agar plate, Agar plate - Preparation of agar plates, Agar plate - Inoculation techniques, Agar plate - Streaking, Agar plate - Christmas tree, Agar plate - Stab culture, Agar plate - Preparing a lawn, Agar plate - Incubation of agar plates, Agar plate - Types of agar plates, Agar plate - Safe disposal of agar plates

Read more here: » Agar plate: Encyclopedia II - Agar plate - Inoculation techniques

Most types of agar are purchased pre-prepared in powder form, although it is possible to buy a base agar mix and add nutrients separately. They are dissolved in distilled water as per their instructions. It is usually necessary to gently boil the mixture to facilitate dissolving: this can be done in a microwave oven, or over a gentle flame. Once dissolved the agar needs to be sterilised, usually by pouring it into a Erlenmeyer flask, then sealing the top with a cotton wool wad, and finally covering the cotton wool with a loose layer of alumi ...

See also:

Agar plate, Agar plate - Preparation of agar plates, Agar plate - Inoculation techniques, Agar plate - Streaking, Agar plate - Christmas tree, Agar plate - Stab culture, Agar plate - Preparing a lawn, Agar plate - Incubation of agar plates, Agar plate - Types of agar plates, Agar plate - Safe disposal of agar plates

Read more here: » Agar plate: Encyclopedia II - Agar plate - Preparation of agar plates

Plates are incubated upside down to prevent drops of condensation from collecting on the inoculated surface. Most plates are incubated at 37° C in a 5% CO2 atmosphere: the temperature and conditions that most of the body's bacteria will grow. Special incubators can maintain these conditions. Some bacteria must be incubated anaerobically (without any oxygen). These can be placed in containers, along with a substance that removes oxygen, and the tightly sealed container placed in the regular incubator. Fungi, and some bacteria (e.g. Yersinia sp.), should be incubated slightly coo ...

See also:

Agar plate, Agar plate - Preparation of agar plates, Agar plate - Inoculation techniques, Agar plate - Streaking, Agar plate - Christmas tree, Agar plate - Stab culture, Agar plate - Preparing a lawn, Agar plate - Incubation of agar plates, Agar plate - Types of agar plates, Agar plate - Safe disposal of agar plates

Read more here: » Agar plate: Encyclopedia II - Agar plate - Incubation of agar plates

Plates, once finished with, must be made safe before throwing away. The usual method is to place inside an autoclave bag and then sterilise by autoclaving at 121 °C, 103 kPa (15 psi) for 15 minutes. Plastic plates will melt (hence the bag). After about 20 minutes the autoclave will have cooled down and the bag can safely be thrown away. If no autoclave is available an ordinary domestic pressure cooker can be used, or, in a hospital or professional lab an incinerator may be used. Other equipment may be decontaminated by being pla ...

See also:

Agar plate, Agar plate - Preparation of agar plates, Agar plate - Inoculation techniques, Agar plate - Streaking, Agar plate - Christmas tree, Agar plate - Stab culture, Agar plate - Preparing a lawn, Agar plate - Incubation of agar plates, Agar plate - Types of agar plates, Agar plate - Safe disposal of agar plates

Read more here: » Agar plate: Encyclopedia II - Agar plate - Safe disposal of agar plates